1/7/2024 0 Comments Rbc normal range![]() They can no longer squeeze into the capillaries, where the main gas exchange occurs therefore, such cells are removed from the circulation in the spleen 5. After changing the RBC’s mechanical phenotype, their functional viability is impaired. These functions are implemented when RBCs penetrate narrow capillaries, the diameter of which is smaller or comparable to the diameter of the RBCs themselves 1 due to their particular biconcave disc shape 2, 3 and specific mechanical properties 4. Developed microfluidic analysis can be used to determine RBCs’ antioxidant capacity for the minimization of anaemia during cancer chemotherapy.ĭelivery of oxygen and nutrients to tissues, removal of CO 2, and metabolic products are provided by specialized blood cells-erythrocytes (red blood cells, RBCs). However, even at high levels of oxidative stress, there are persistent cells in the population with an undisturbed biophysical phenotype that retain the ability to move in microcapillaries. It leads to cell swelling, increased stiffness and adhesion, resulting in a decrease in the transit velocity in microcapillaries. The data indicates that the built-in antioxidant defence system has a limit exceeding which haemoglobin oxidation, membrane, and cytoskeleton transformation occurs. However, single-cell tracking combined with cytological and AFM studies reveals cell heterogeneity, which increases with the level of oxidative stress. ![]() Oxidative stress was expected to make RBCs more rigid, which would lead to decrease their transit velocity in microfluidic channels. Here, we used microfluidics to simulate the microcirculation of RBCs under oxidative stress induced by tert-Butyl hydroperoxide. It occurs during numerous pathological processes and causes anaemia, one of the most frequent side effects of cancer chemotherapy. Oxidative stress is one of the key factors that leads to red blood cells (RBCs) aging, and impairs their biomechanics and oxygen delivery.
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